个人简介：

尹新坚，副教授，硕士生导师。2019年毕业于浙江大学，获工学博士学位。主要研究方向为海洋酶催化技术开发：1.海洋新颖酶催化元件挖掘：采用基因组探矿、宏基因组挖掘等技术从海洋资源中筛选具有工业应用潜力的新型海洋酶催化剂。2.海洋酶蛋白分子改造：针对海洋酶蛋白特性，建立高效分子改造技术，增强其催化活力、温度稳定性、立体选择性、辅酶特异性等属性。3.海洋酶催化剂的应用：开发高效海洋酶催化技术，将其应用于化学品合成、食品加工处理、海洋环境保护等领域。以第一或通讯作者身份在ACS Catalysis、Journal of Agricultural and Food Chemistry、International Journal of Biological Macromolecules、Bioorganic Chemistry等本领域权威期刊发表多篇。主持国家自然科学面上项目等省部级以上项目5项。获授权美国发明专利1项、欧洲发明专利1项、中国发明专利10余项。

 

工作经历：

  • 2025年11月-至今，中山大学海洋科学学院，副教授，硕士生导师
  • 2022年8月-2025年10月，中山大学海洋科学学院，特聘副研究员
  • 2019年7月-2022年7月，中山大学海洋科学学院，博士后

 

主持科研项目：

  • 国家自然科学基金-面上项目， 主持，2026-2029， 50 万元
  • 国家自然科学基金-青年项目，主持，2022-2024， 30 万元
  • 博士后科学基金-面上项目（一等），主持，2021-2022， 12 万元
  • 广东省自然科学基金-面上项目，主持，2021-2023， 10 万元
  • 广东省自然科学基金-青年基金，主持，2020-2022， 10 万元

 

代表性研究论文：

1.Xinjian Yin, Wenzhong Gong, Yujing Zeng, Hulin Qiu, Lan Liu, Frank Hollmann,* and Bishuang Chen*. Substrate-Specific Evolution of Amine Dehydrogenases for Accessing Structurally Diverse Enantiopure (R)‑β-Amino Alcohols, ACS Catalysis, 2024, 14, 837-845. (IF=13.1)

2. Hulin Qiu#,Wenzhong Gong#, Huilin Huang, Heng Guo, ZhizengGao, Lan Liu, Xinjian Yin*. Rosetta-Driven Rapid Evolution of Meso-Diaminopimelate Dehydrogenases for Structurally Diverse D-amino Acid Production, Journal of Agricultural and Food Chemistry ,2025, 73, 25, 15847–15859. ( IF=6.2)

3.Yongyi Zeng#, Xinjian Yin# , Yating Zou, YitingSu, Lan Liu, Bishuang Chen*, Xingcai Zhang. Magnetic multifunctional module multiphasic maneuver mimicking multienzyme magic (M8), Journal of Agricultural and Food Chemistry ,2025,73, 27,17128-17137. ( IF=6.2)

4.Yujing Zeng#, Keke Zhang#, Tiantian Lu#, Xinjian Yin#, Lan Liu*, Honglei Ma*, Zhizeng Gao* et al, Structural and Mechanistic Basis for Nitrile Synthetase by an Argininosuccinate Synthetase-Like Enzyme. ACS Catalysis., 2025, 15,16254-16267.( IF=13.1)

5.Guosi Li, Shanyong Yi, Haijiao Wang, Hulin Qiu, Wei Wang, Leilei Gao, Qilin Xu, Bangxing Han*, Xinjian Yin*; Salidroside Production through Cascade Biocatalysis with a Thermostability-Enhanced UDP-Glycosyltransferase, International Journal of Biological Macromolecules，2025, 299: 140261.( IF=8.5)

6.Jiayan Du, Youtong Bao, Jingyuan Zhu, Xueqing Pang, Depeng Ren, Xinjian Yin*, Pingping Zhou*, Engineering of β-carotene hydroxylase for enhanced astaxanthin production in Saccharomyces cerevisiae, Biochemical Engineering Journal, 2025, 219, 109722. (IF=3.8)

7.Guosi Li, Wei Wang, Heng Guo, Shanyong Yi, Fang Wang, Shiping Huang, Nan Hu, Qilin Xu, Yongjun Zang, Bangxing Han, Xinjian Yin*. Mutability landscape guided engineering of a promiscuous microbial glycosyltransferase for regio-complementary synthesis of glucosides, International Journal of Biological Macromolecules, 2024, 263, 130229. ( IF=8.5)

8.Xiuqi Zhou, Jiayan Du, Jinyuan Zhu, Xueqing Pang, Xinjian Yin*, Pingping Zhou*. Structure-guided engineering of 4-coumarate: CoA ligase for efficient production of rosmarinic acid in Saccharomyces cerevisiae, Journal of Biotechnology, 2024, 396, 140-149. (IF=3.9)

9.Xinjian Yin, Yujing Zeng, Jun Chen, Lan Liu, Zhizeng Gao*. Combined active pocket and hinge region engineering to develop an NADPH-dependent phenylglycine dehydrogenase, Bioorganic Chemistry,2022. 120,105601. (IF=4.7)

10.Xinjian Yin, Wenzhong Gong, Zhigeng Zhan, Wei Wei, Mengmeng Li, Jianyu Jiao, Bishuang Chen, Lan Liu, Wenjun Li*, Zhizeng Gao*. Mining and engineering of valine dehydrogenases from a hot spring sediment metagenome for the synthesis of chiral non-natural L-amino acids, Molecular catalysis,2022. 533,112767. (IF=4.9)

11.Guosi Li, Fucheng Zhu, Peipei Wei, HailongXue, Naidong Chen, Baowei Lu, Hui Deng, Cunwu Chen*, Xinjian Yin*. Metabolic Engineering of Escherichia coli for Hyperoside Biosynthesis, Microorganisms, 2022. 10(3), 628. (IF=4.2)

12.Xinjian Yin, Litong Chen, Siwen Yuan, Lan Liu, Zhizeng Gao*. A robust high-throughput fluorescent polarization assay for the evaluation and screening of SARS-CoV-2 fusion inhibitors, Bioorganic Chemistry,2021.116, 105362. (IF=4.7)

13.Xinjian Yin, Yayun Liu, Lijun Meng, Haisheng Zhou, Jianping Wu*; Lirong Yang*. Semi-rational Hinge Engineering: Modulating the Conformational Transformation of Glutamate Dehydrogenase for Enhanced Reductive Amination Activity towards Non-natural Substrates, Catalysis Science & Technology, 2020, 10: 3376-3386. (IF=4.2)

14.Xinjian Yin ,Jiaxin Li, Senhua Chen, Yuping Wu, Zhigang She, Lan Liu, Yue Wang*, Zhizeng Gao*. An Economical High‐Throughput “FP‐Tag” Assay for Screening Glycosyltransferase Inhibitors, ChemBioChem, 2020, 22: 1-6,(IF=2.8)

15.Xinjian Yin, Yayun Liu, Lijun Meng, Haisheng Zhou, Jianping Wu, Lirong Yang*. Rational Molecular Engineering of Glutamate Dehydrogenases for Enhancing Asymmetric Reductive Amination of Bulky alpha-Keto Acids, Advanced Synthesis & Catalysis, 2019, 361(4): 803-812. (IF=4.0)

16.Xinjian Yin, Jianping Wu, Lirong Yang*. Efficient Reductive Amination Processfor Enantioselective Synthesis of L-Phosphinothricin Applying Engineered Glutamate Dehydrogenase, Applied Microbiology and Biotechnology, 2018, 102(10): 4425-4433. (IF=4.3)

 

授权发明专利：

1. Glutamate dehydrogenase mutant and use thereof in preparing L-glufosinate. 欧洲专利，EP3677673. -授权

2.Glutamate dehydrogenase mutants and their application in preparation of L-phosphinothricin. 美国专利， US 10865391. -授权

3.《海洋宏基因组来源内消旋-二氨基庚二酸脱氢酶突变体及其在D-色氨酸合成中的应用》，专利申请号：202510179757.X. –授权

4.《缬氨酸脱氢酶及其突变体在制备手性L-氨基酸中的应用》，专利申请号：202210976935.8. –授权

5.《工程化NADPH依赖型苯甘氨酸脱氢酶及其应用》，专利申请号：2021115422862. –授权

6.《D-氨基酸脱氢酶突变体及其在手性D-氨基酸合成中的应用》，专利申请号：202411879696X. –授权

7.《一种糖基转移酶突变体及其在红景天苷合成中的应用》，专利申请号：2023111711877. –授权

8.《一种糖基转移酶突变体及酶法制备急性缺血性脑卒中候选药物SHPL-49的方法》，专利申请号：2023117640748. –授权

9.《谷氨酸脱氢酶突变体及其应用》，专利申请号：2019104033312. –授权

10.《谷氨酸脱氢酶突变体及其在制备L-草铵膦中的应用》，专利申请号：2018101947420.–授权

11.《一种利用氨基酸脱氢酶制备L-草铵膦的方法》，专利申请号：2017102100309. –授权